3) the protein is from animals – not plants
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Because plant proteins are cheaper than meat proteins, pet food companies will have a higher profit margin when using corn, wheat, soy, rice, etc.
Similar to TnaC described above, the peptide SecM exists solely to stallthe ribosome synthesizing it. But unlike TnaC, which also requires thepresence of high levels of trytophan, SecM has an intrinsic stallingcapability. Stalling of the ribosome synthesizing SecM provides time fora downstream RNA helix on the same mRNA strand to unwind. Unwinding ofthis helix then allows for a new ribosome to bind and synthesize anew protein, SecA, a bacterial ATP-driven translocase that aids the passage ofnascent proteins across membranes in conjunction with SecY (see also ). When sufficient levels of SecA have been reached,SecA interacts with the SecM-stalled ribosome to pull on SecM, freeingit and allowing translation to resume (illustrated schematically inFig. 13). SecM, which serves no otherpurpose than to stall the ribosome, is released into the cell anddegraded.
Transcription is the first step in protein synthesis.
Working principle of common methods to isolate extracellular vesicles (EVs). Separation is based on size, density, and immunophenotype. Straight brackets: isolated EVs; yellow: soluble components; and blue: buffer. A, In differential centrifugation, separation is based on size, and large EVs (gray) collect earlier at the bottom of the tube and at lower g forces than small EVs (green). The soluble components are not affected by centrifugation, but non-EV particles such as lipoproteins and protein aggregates may copellet with EVs. B, In density gradient centrifugation, separation is based on density, and EVs will travel to their equilibrium density. Non-EV particles such as lipoproteins may coelute with EVs because of similar density or interaction. The soluble components with a high density relative to the gradient will collect at the bottom of the tube. C, Size exclusion chromatography uses a porous matrix (dotted circles) that separates on size. Soluble components and particles smaller than the size cutoff enter the porous matrix temporarily, whereas EVs and particles larger than the size cutoff do not enter the porous matrix. As a result, EVs and particles larger than the size cutoff elute before the soluble components and particles smaller than the size cut-off. D, In ultrafiltration, soluble proteins and particles smaller than the size cutoff (≈105 kDa) are pushed through the filter, and the EVs are collected at the filter. E, In immunocapture assays, EVs are captured based on their immunophenotype. EVs are captured using an monoclonal antibody (mAb) directed against an antigen exposed on the targeted (green) EVs only. F, In precipitation, addition of a precipitating agent induces clumping of EVs, non-EV particles, and soluble proteins. The clumps will sediment, and sedimentation can be accelerated by centrifugation. 
Biogenesis of exosome. Exosomes are compartmentalized into the multivesicular bodies (MVBs), which fuse with the cell membrane and released to the extracellular space. This process prevents exosomes from degradation by the lysosomes. Exosomes comprise of various transmembrane and cytosolic proteins, such as integrins, CD9, CD63, and CD81. Furthermore, exosomes retain donor cells’ proteins, DNA fragments, miRNAs, and noncoding RNAs within the bilipid membrane. As such, exosomes preserve the genetic information of donor cells from enzymatic degradation while enabling targeted delivery of specific cargo. 
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Feline Asthma/Allergic Airway Disease: Many cats have had their respiratory symptoms (coughing/difficulty breathing) subside considerably, or disappear completely, once they were placed on a grain-free canned food diet, or a meat-based homemade diet. Some of these struggling cats may have been reacting to storage mites or cockroach antigens that are present in dry foods, or they may have been reacting to the gluten (protein fraction) part of the grains that are present in dry foods.
Feeding a high-protein, low-carbohydrate canned diet helps keep cats at an optimal, healthy body weight and, in turn, makes them less likely to end up with fatty liver disease.
RCSB Protein Data Bank - RCSB PDB
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A peptide chain is a newly formed protein made up of amino acids covalently bonded by peptide bonds.
Portion sizes of protein rich foods
coli Ras-like Protein
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subtilis Secretory Protein
Protein Synthesis facts, information, pictures m
Cats are obligate (strict) carnivores and are very different from dogs in their nutritional needs. What does it mean to be an ‘obligate carnivore’? It means that your cat was built by Mother Nature to get her nutritional needs met by the consumption of a large amount of animal-based proteins (meat/organs) and derives much less nutritional support from plant-based proteins (grains/vegetables). It means that cats lack specific metabolic (enzymatic) pathways and cannot utilize plant proteins as efficiently as animal proteins.
Lab 17a: Simulating Protein Synthesis with Pictures
Mechanisms involved in the modulation of Angiogenesis by endothelial cell (EC)–derived extracellular vesicles (EVs). EC release EVs rich in micro-RNA such as miR-214 and miR-126, that are transferred to recipient EC and induce proangiogenic signaling. EVs contain functional matrix metalloproteinases that facilitate angiogenesis through the degradation of components of the extracellular matrix. Dll4 is transferred to EC by the EVs and induces Notch receptor internalization and tip cell formation. EVs bear, at their surface, a tissue factor that interacts with β1 integrin and induces Rac1-ERK1/2-ETS1 signaling, leading to the increased secretion of CCL2. EVs transport the complex uPA/uPAR, which stimulates angiogenesis through plasmin generation. The phosphatidylserine present on the surface of the EVs interacts with CD36 and induces Fyn kinase signaling, which leads to increased oxidative stress and the inhibition of angiogenesis. ATM indicates ataxia telangiectasia mutated; CCl2, chemokine c-c motif ligand 2; Dll4, Delta-like 4; ECM, extracellular matrix; ERK1/2, extracellular signal-related kinase 1 and 2; ETS1, avian erythroblastosis virus E26 homolog-1; IL-3R, interleukin-3 receptor; MMPs, matrix metalloproteinases; NotchR, Notch receptor; PS, phosphatidylserine; Rac1, Ras-related C3 botulinum toxin substrate 1; ROS, reactive oxygen species; TF, tissue factor; TIMPS, tissue inhibitor of metalloproteinases; uPA, urokinase plasminogen activator; and uPAR, urokinase plasminogen activator receptor. 
What Is Muscle Protein Synthesis? (with pictures)
Proteins derived from animal tissues have a complete amino acid profile. (Amino acids are the building blocks of proteins. Think of them as pieces of a puzzle.) Plant-based proteins do not contain the full complement (puzzle pieces) of the critical amino acids required by an obligate carnivore. The quality and composition of a protein (are all of the puzzle pieces present?) is also referred to as its biological value.
Protein Synthesis - Estrella Mountain Community College
Humans and dogs can take the pieces of the puzzle in the plant protein and, from those, make the missing pieces. Cats cannot do this. This is why humans and dogs can live on a vegetarian diet but cats cannot. (Note that I do not recommend vegetarian diets for dogs.)
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